Influenza pandemics throughout Brazilian history.

Brazil has experienced several major epidemics of influenza, and the most destructive was in 1918-1919. This article focuses on mortality, mitigation policies, and the consequences of pandemic influenza during the national period. We provide the first mortality estimates for the 1890-1894 influenza pandemic and correct figures for later epidemics. The 1918-1919 episode cost more lives than assumed, although some cities suffered less, possibly because of public health actions. Influenza caused pandemics in 1957, 1968, 1976, and 2009, but these did not cause unusual outbreaks in Brazil.

Influenza pandemics throughout Brazilian history / Pandemias de influenza ao longo da história brasileira

Abstract Brazil has experienced several major epidemics of influenza, and the most destructive was in 1918-1919. This article focuses on mortality, mitigation policies, and the consequences of pandemic influenza during the national period. We provide the first mortality estimates for the 1890-1894 influenza pandemic and correct figures for later epidemics. The 1918-1919 episode cost more lives than assumed, although some cities suffered less, possibly because of public health actions. Influenza caused pandemics in 1957, 1968, 1976, and 2009, but these did not cause unusual outbreaks in Brazil.

Resumo O Brasil passou por várias epidemias importantes de influenza, a mais letal em 1918-1919. O artigo focaliza a mortalidade, as políticas de mitigação e as consequências das pandemias de influenza no período nacional. Fornecemos as primeiras estimativas de mortalidade para a pandemia de 1890-1894 e corrigimos números de epidemias posteriores. O episódio de 1918-1919 custou mais vidas do que se considerou anteriormente, embora algumas cidades tenham sofrido menos, possivelmente devido a ações de saúde pública. A influenza gerou pandemias em 1957, 1968, 1976 e 2009, mas elas não causaram surtos incomuns no Brasil.

Leviathan as a partial cure? Opportunities and pitfalls of using the state-owned apparatus to respond to the COVID-19 crisis / ¿Leviatán como una cura parcial? Oportunidades y dificultades al usar el aparato estatal para responder a la crisis de COVID-19 / O Leviatã como uma cura parcial? Oportunidades e armadilhas no uso do aparato estatal para responder à crise da COVID-19

Abstract While some argue that market forces are imperative to stimulate the increased supply of critical products and services to address the COVID-19 crisis, others contend that dealing with the pandemic requires coordination and rapid adjustments in supply that may be constrained by a host of factors. Although discussions have centered on policies to promote financial liquidity, we examine whether the state apparatus-not only state-owned enterprises but also development agencies and investment funds-can innovate and adjust production processes to improve infrastructure and capabilities to prevent and treat the disease. Potential actions include public-private effort addressing both discovery and coordination problems-such as a collaborative effort to develop prevention and treatment technologies, as well as state capital to stimulate retooling and expansion of strategic infrastructure. The state apparatus can also help access remote and critical areas with relatively lower private returns. In contrast, support to industries must be implemented with caution, especially for sectors whose demand may suffer permanently due to lifestyle changes. Exit strategies must be carefully crafted to avoid the risk of perpetuating unjustified and ineffective state support, establishing milestones and termination clauses based on clear performance indicators. We argue that, although using the state apparatus as a countermeasure entails a set of risks, not using it may increase the risk of extending the crisis and end with an overloaded state sector (e.g., due to massive bailouts), challenging the implementation of subsequent adjustments.

Resumen Mientras que algunos sostienen que las fuerzas del mercado son imperativas para estimular el suministro de productos y servicios críticos para enfrentar la crisis de COVID-19, otros recomiendan coordinación y ajustes rápidos en el suministro que pueden estar limitados por varios factores. Si bien las discusiones habituales se centran en políticas para promover la liquidez financiera, examinamos si el aparato estatal ‒no solo las empresas estatales, sino también las agencias de desarrollo y los fondos públicos‒ puede promover la experimentación y el ajuste en los procesos de producción. Las acciones potenciales incluyen esfuerzos público-privados que buscan el descubrimiento y la coordinación, como los esfuerzos de colaboración para desarrollar tecnologías de prevención y tratamiento, así como inyecciones de capital para estimular la expansión de la infraestructura estratégica. El aparato estatal también puede ayudar a acceder a áreas remotas y críticas, con rendimientos privados más bajos. Por otro lado, el apoyo selectivo a las industrias debe implementarse con precaución, especialmente en sectores cuya demanda puede sufrir cambios permanentes debido a un nuevo estilo de vida. Para evitar el riesgo de perpetuar el apoyo estatal injustificado, las estrategias de salida deben diseñarse cuidadosamente, con hitos y cláusulas de terminación basadas en indicadores de desempeño. Aunque usar el aparato estatal como contramedida conlleve su propio conjunto de riesgos, quizás paradójicamente no usarlo también aumente el riesgo de extender la crisis y de dar como resultado un sector estatal hinchado (por ejemplo, debido a rescates masivos), lo que dificulta la implementación de ajustes posteriores.

Resumo Enquanto alguns argumentam que as forças de mercado são imperativas para estimular a oferta de produtos e serviços críticos para lidar com a crise da COVID-19, outros recomendam coordenação e ajustes rápidos na oferta que podem ser limitados por uma série de fatores. Embora as discussões usuais coloquem foco em políticas para promover liquidez financeira, examinamos se o aparato estatal - não apenas empresas estatais, mas também agências de desenvolvimento e fundos públicos - pode promover experimentação e ajuste nos processos de produção para aumentar a infraestrutura e capacidade de prevenção e tratamento. Ações potenciais incluem esforços público-privados buscando descoberta e coordenação - como esforços colaborativos para desenvolver tecnologias de prevenção e tratamento, além de injeções de capital para estimular o reequipamento e a expansão da infraestrutura estratégica. O aparato estatal também pode ajudar a acessar áreas remotas e críticas, com retornos privados relativamente mais baixos. Por outro lado, apoio seletivo às indústrias deve ser implementado com cautela, especialmente em setores cuja demanda pode sofrer alterações permanentes devido a mudanças no estilo de vida. Para evitar o risco de perpetuar apoio estatal injustificado e ineficaz, estratégias de saída devem ser elaboradas com cuidado, com marcos e cláusulas de término com base em indicadores de desempenho. Embora o uso do aparato estatal como contramedida acarrete seu próprio conjunto de riscos, talvez paradoxalmente não utilizá-lo também aumente o risco de estender a crise e acabar com um setor estadual inchado (por exemplo, devido a resgates maciços), tornando mais difícil a implementação de ajustes subsequentes.

Differences in pollen viability in relation to different deceptive pollination strategies in Mediterranean orchids.

BACKGROUND AND AIMS: To date, current research involving pollen viability has been evaluated in a relatively low number of orchid species. In the present study, we focused on five related Mediterranean orchid genera (Anacamptis, Orchis, Dactylorhiza, Ophrys and Serapias) that are characterized by different types of deceptive pollination. METHODS: The in vitro germination ability of increasingly aged pollinaria of eight food-, seven sexually and two shelter-deceptive species was evaluated. Pollination experiments on two food-, one sexually and one shelter-deceptive species were also performed and the percentage of embryonate seeds derived from the increasingly aged pollinaria was checked. KEY RESULTS: All of the examined species showed long-term viabilities (=50 % pollen tube growth) that ranged from 8 to 35 d. Species with the same deceptive pollination strategies exhibited the same pollen viability trends. Interestingly, pollen viabilities of species groups with different deception types have shown significant differences, with sexually and shelter- deceptive species exhibiting a shorter life span than food-deceptive species. CONCLUSIONS: This study confirms the prolonged germination and fertilization capacities of orchid pollinaria, and to our knowledge is the first report demonstrating a clear relationship between pollen viability and pollination system. It is proposed that this relationship is attributed to the different types of reproductive barriers, pre- or post-zygotic, that characterize Ophrys and Serapias and the food-deceptive species, respectively.

Strong post-pollination pre-zygotic isolation between sympatric, food-deceptive Mediterranean orchids.

Mediterranean orchids that grow in admixed, co-flowering populations, and frequently show hybrid progenies are interesting to use to study the nature and the strength of post-zygotic barriers. However, examination of pre- and post-pollination pre-zygotic isolating mechanisms requires sympatric, co-flowering species pairs that do not produce hybrid swarms. In this study, we analyzed a contact zone between Orchis italica and O. papilionacea, in which hybrid forms have never been reported, although hybridization between members of their groups of appurtenance has been signaled. We investigated pre-pollination barriers observing the floral phenology of both species and identified pollinators by means of molecular analysis of pollinaria collected on the insects captured in the study site. Post-pollination barriers were tested performing manual crosses in order to evaluate pollen germination/pollen tube growth in vivo and fruit and seed formation. Floral phenologies of O. italica and O. papilionacea display nearly overlapping trends, and two common pollinators have been identified by molecular analysis of pollinaria. Thus, pre-pollination barriers are very weak or nonexistent. Bidirectional crosses have shown that the growth of heterospecific pollen tubes is fully blocked in stigmatic cell layers. Since no fruit formation was detected in bidirectional interspecific crosses, we assume that reproductive isolation between the examined species is fully guaranteed by post-pollination pre-zygotic mechanisms acting at stigmatic level. Such condition has been rarely described and may mask the potential action of post-zygotic mechanisms.

Contrasting thoughts about deceptive orchids: a response to Sobel and Randle.

Sobel and Randle (2009) challenge several methodological choices in the comparative study of the evolution of reproductive isolation in Mediterranean deceptive orchids of Scopece et al. (2007) including the species concept used and the selection of taxa, together with the perceived comparison of clades of different ages. They further criticize that pollinator information was taken from the literature and that two different methods were used to estimate pollinator specificity in food-deceptive and sexually deceptive orchids, respectively. Here we reply to these challenges.

Patterns of reproductive isolation in Mediterranean deceptive orchids.

The evolution of reproductive isolation is of central interest in evolutionary biology. In plants, this is typically achieved by a combination of pre- and postpollination mechanisms that prevent, or limit, the amount of interspecific gene flow. Here, we investigated and compared two ecologically defined groups of Mediterranean orchids that differ in pollination biology and pollinator specificity: sexually deceptive orchids versus food-deceptive orchids. We used experimental crosses to assess the strength of postmating prezygotic, and postzygotic reproductive isolation, and a phylogenetic framework to determine their relative rates of evolution. We found quantitative and qualitative differences between the two groups. Food-deceptive orchids have weak premating isolation but strong postmating isolation, whereas the converse situation characterizes sexually deceptive orchids. Only postzygotic reproductive isolation among food-deceptive orchids was found to evolve in a clock-like manner. Comparison of evolutionary rates, within a common interval of genetic distance, showed that the contribution of postmating barriers was more relevant in the food-deceptive species than in the sexually deceptive species. Asymmetry in prezygotic isolation was found among food-deceptive species. Our results indicate that postmating barriers are most important for reproductive isolation in food-deceptive orchids, whereas premating barriers are most important in sexually deceptive orchids. The different rate of evolution of reproductive isolation and the relative strength of pre- and postmating barriers may have implication for speciation processes in the two orchid groups.

Hypervariable plastid locus variation and intron evolution in the Anacamptis palustris lineage.

Data on the organization of a hypervariable chloroplast locus in the Anacamptis palustris (Orchidaceae) lineage are provided and used to infer the pattern of molecular evolution in this group. A large survey of sequence variation in A. palustris and allied taxa reveals several repeat types differing in number and organization that occur in the same plastid region. The resulting repeat type network suggests that at least seven different minisatellite loci evolved near each other in the tRNALEU intron and indicates the presence of at least three main phyletic lines in the A. palustris lineage.

Molecular evolution of a plastid tandem repeat locus in an orchid lineage.

The molecular evolution of a chloroplast minisatellite locus in the Anacamptis palustris (Orchidaceae) lineage and haplotype variation in two Italian A. palustris populations were investigated. A phylogenetic analyses of the chloroplast tRNA(LEU) intron, where the minisatellite locus is located, revealed that a deletion in the ancestor of the A. palustris lineage led to the formation of two noncontiguous, complementary sequence motifs. We propose a model to explain the initial formation of the minisatellite repeat motif, starting with the two noncontiguous, complementary sequence motifs. A survey of minisatellite variation in four species of the A. palustris lineage revealed several haplotypes that differed not only in repeat number, but also in repeat organization. A haplotype network suggests that three different minisatellite loci evolved independently at the same position in the tRNA(LEU) intron. A secondary structure model revealed that the A. palustris minisatellite repeat forms a stem region of the tRNA(LEU) intron, which allows its notable expansion without negatively affecting splicing. Minisatellite variation was high in the two examined A. palustris populations where 20 haplotypes were detected, whereas no length variation was detected in a neighboring poly (A) microsatellite locus. We estimated a chloroplast minisatellite mutation rate of 3.2 x 10(-3) mutations per generation. Southern blot analyses did not find evidence for chloroplast heteroplasmy. Based on the analysis of the largest known, extant A. palustris population, a stepwise mutation model (SMM) was inferred.

Variation at a chloroplast minisatellite locus reveals the signature of habitat fragmentation and genetic bottlenecks in the rare orchid Anacamptis palustris (Orchidaceae).

Geoclimatic changes during the Oligocene and more recent anthropogenic influences have shaped the current distribution and population structure of Mediterranean plant species. Anacamptis palustris (Orchidaceae) is a typical member of coastal wetlands, which have become increasingly fragmented and isolated. As a consequence, this orchid has become rare in the recent past. Length variation at a chloroplast minisatellite locus was used to estimate genetic variation within and between the largest extant populations of A. palustris. Genetic diversity was positively correlated with population size. Estimation of observed and expected gene diversity and analyses of haplotype number and haplotype frequency distributions provided evidence for population bottlenecks in the history of small populations. Comparison with an earlier study suggests that nuclear allozyme diversity was most likely lost during the Oligocene and could not recover subsequently due to low mutation rates, whereas genetic variation was restored at the highly variable chloroplast minisatellite locus. Population bottlenecks indicated by cpDNA variation occurred most likely as a consequence of more recent anthropogenic changes. The comparison of molecular markers with different levels of polymorphism provided valuable insights into the processes shaping genetic diversity and population structure in this rare orchid.